Analysis of Stem Cell Culture Performance in a Microcarrier Bioreactor System
نویسندگان
چکیده
An analysis of the effects of the turbulent shear stress on cell culture in a stirred tank microcarrier bioreactor system using a synergistic combination of computational fluid dynamic (CFD)-based simulations and experiments is presented. A 3D computational model of Corning’s benchscale spinner flask was built using a state-of-the-art computational fluid dynamics (CFD) software, Flow3D (www.flow3d.com). The effects of parameters such as the impeller speed, culture medium fluid properties and particle size on the steady-state shear stress acting on the cell-laden microcarrier particles in the bioreactor are studied using CFD analysis. This is used to predict the precise shear conditions experienced by cells and identify optimum operating conditions that prevent turbulent shear damage of the cells. In addition, the effect of shear on the pluripotency of hPSCs is studied by determining the percentage of cells carrying the pluripotency markers Oct4, Sox2 and Nanog using flow cytometry and quantitative PCR.
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